Coding

Part:BBa_K108020

Designed by: Qi Liu   Group: iGEM08_Tsinghua   (2008-10-23)

Dam

DNA adenine methylase of E.coli

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Lucas Ng
  • Summary: Added information collated from existing scientific studies

dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [1].

It is likely to act in postreplication mismatch repair [2] rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family [3].

Furthermore, it may also be involved in DNA replication [4]

Reaction

a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine [5]

References

  1. Geier, G. E., and P. Modrich. 1979. “Recognition Sequence of the Dam Methylase of Escherichia Coli K12 and Mode of Cleavage of Dpn I Endonuclease.” The Journal of Biological Chemistry 254 (4): 1408–13. https://pubmed.ncbi.nlm.nih.gov/368070.
  2. Lu, A. L., S. Clark, and P. Modrich. 1983. “Methyl-Directed Repair of DNA Base-Pair Mismatches in Vitro.” Proceedings of the National Academy of Sciences 80 (15): 4639–43. https://doi.org/10.1073/pnas.80.15.4639.
  3. Horton, John R., Xing Zhang, Robert M. Blumenthal, and Xiaodong Cheng. 2015. “Structures of Escherichia Coli DNA Adenine Methyltransferase (Dam) in Complex with a Non-GATC Sequence: Potential Implications for Methylation-Independent Transcriptional Repression.” Nucleic Acids Research 43 (8): 4296–4308. https://doi.org/10.1093/nar/gkv251.
  4. Smith, D. W., A. M. Garland, G. Herman, R. E. Enns, T. A. Baker, and J. W. Zyskind. 1985. “Importance of State of Methylation of OriC GATC Sites in Initiation of DNA Replication in Escherichia Coli.” The EMBO Journal 4 (5): 1319–26. https://pubmed.ncbi.nlm.nih.gov/3891329.
  5. “Rhea - Annotated Reactions Database.” n.d. Rhea. Accessed July 26, 2021. https://www.rhea-db.org/rhea/15197.
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Categories
//cds/enzyme/methylation
Parameters
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